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Procell Inc human ovarian carcinoma cell line skov3
Human Ovarian Carcinoma Cell Line Skov3, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human ovarian carcinoma cell line skov3/product/Procell Inc
Average 90 stars, based on 1 article reviews
human ovarian carcinoma cell line skov3 - by Bioz Stars, 2026-03
90/100 stars

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ATCC skov3 human ovarian carcinoma cell line
6-gingerol induces apoptosis in <t>SKOV3</t> cells. (a) Clonogenic survival assay showing the survival rates of SKOV3 cells treated with 5 µM,10 µM,15 µM and 20 µM 6-gingerol for different durations (1 st , 2 nd , 4 th , and 6 th days). Results are based on independent experiments (n = 3). (b) Western blot analysis of cleaved caspase-3 or and cleaved PARP levels in SKOV3 cells treated with 6-gingerol. Tubulin was used as the loading control. (c) Flow cytometry analysis of apoptosis in SKOV3 cells treated with different 6-gingerol concentrations, using an Annexin V-FITC & propidium iodide (PI) apoptosis kit. Results are from three independent experiments (n = 3). (d) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel (c) . Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.001. (e) Flow cytometry analysis of apoptosis in SKOV3 cells treated with 20 µM Z-VAD-FMK (Selleck Chemicals) for 2 hours, followed by treatment with 20 µM 6-gingerol and treated with 20 µM 6-gingerol directly for 2 days, using an Annexin V-FITC & propidium iodide (PI) apoptosis kit. Results are from three independent experiments (n = 3). (f) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel **P < 0.01, ***P < 0.001 (e) . Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.001.
Skov3 Human Ovarian Carcinoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/skov3 human ovarian carcinoma cell line/product/ATCC
Average 99 stars, based on 1 article reviews
skov3 human ovarian carcinoma cell line - by Bioz Stars, 2026-03
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90
China Center for Type Culture Collection human serous epithelial ovarian carcinoma cell line skov3
6-gingerol induces apoptosis in <t>SKOV3</t> cells. (a) Clonogenic survival assay showing the survival rates of SKOV3 cells treated with 5 µM,10 µM,15 µM and 20 µM 6-gingerol for different durations (1 st , 2 nd , 4 th , and 6 th days). Results are based on independent experiments (n = 3). (b) Western blot analysis of cleaved caspase-3 or and cleaved PARP levels in SKOV3 cells treated with 6-gingerol. Tubulin was used as the loading control. (c) Flow cytometry analysis of apoptosis in SKOV3 cells treated with different 6-gingerol concentrations, using an Annexin V-FITC & propidium iodide (PI) apoptosis kit. Results are from three independent experiments (n = 3). (d) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel (c) . Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.001. (e) Flow cytometry analysis of apoptosis in SKOV3 cells treated with 20 µM Z-VAD-FMK (Selleck Chemicals) for 2 hours, followed by treatment with 20 µM 6-gingerol and treated with 20 µM 6-gingerol directly for 2 days, using an Annexin V-FITC & propidium iodide (PI) apoptosis kit. Results are from three independent experiments (n = 3). (f) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel **P < 0.01, ***P < 0.001 (e) . Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.001.
Human Serous Epithelial Ovarian Carcinoma Cell Line Skov3, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human serous epithelial ovarian carcinoma cell line skov3/product/China Center for Type Culture Collection
Average 90 stars, based on 1 article reviews
human serous epithelial ovarian carcinoma cell line skov3 - by Bioz Stars, 2026-03
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ATCC skov3 ddp human ovarian carcinoma cell lines
6-gingerol induces apoptosis in <t>SKOV3</t> cells. (a) Clonogenic survival assay showing the survival rates of SKOV3 cells treated with 5 µM,10 µM,15 µM and 20 µM 6-gingerol for different durations (1 st , 2 nd , 4 th , and 6 th days). Results are based on independent experiments (n = 3). (b) Western blot analysis of cleaved caspase-3 or and cleaved PARP levels in SKOV3 cells treated with 6-gingerol. Tubulin was used as the loading control. (c) Flow cytometry analysis of apoptosis in SKOV3 cells treated with different 6-gingerol concentrations, using an Annexin V-FITC & propidium iodide (PI) apoptosis kit. Results are from three independent experiments (n = 3). (d) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel (c) . Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.001. (e) Flow cytometry analysis of apoptosis in SKOV3 cells treated with 20 µM Z-VAD-FMK (Selleck Chemicals) for 2 hours, followed by treatment with 20 µM 6-gingerol and treated with 20 µM 6-gingerol directly for 2 days, using an Annexin V-FITC & propidium iodide (PI) apoptosis kit. Results are from three independent experiments (n = 3). (f) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel **P < 0.01, ***P < 0.001 (e) . Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.001.
Skov3 Ddp Human Ovarian Carcinoma Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/skov3 ddp human ovarian carcinoma cell lines/product/ATCC
Average 99 stars, based on 1 article reviews
skov3 ddp human ovarian carcinoma cell lines - by Bioz Stars, 2026-03
99/100 stars
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90
Procell Inc human ovarian carcinoma cell line skov3
6-gingerol induces apoptosis in <t>SKOV3</t> cells. (a) Clonogenic survival assay showing the survival rates of SKOV3 cells treated with 5 µM,10 µM,15 µM and 20 µM 6-gingerol for different durations (1 st , 2 nd , 4 th , and 6 th days). Results are based on independent experiments (n = 3). (b) Western blot analysis of cleaved caspase-3 or and cleaved PARP levels in SKOV3 cells treated with 6-gingerol. Tubulin was used as the loading control. (c) Flow cytometry analysis of apoptosis in SKOV3 cells treated with different 6-gingerol concentrations, using an Annexin V-FITC & propidium iodide (PI) apoptosis kit. Results are from three independent experiments (n = 3). (d) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel (c) . Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.001. (e) Flow cytometry analysis of apoptosis in SKOV3 cells treated with 20 µM Z-VAD-FMK (Selleck Chemicals) for 2 hours, followed by treatment with 20 µM 6-gingerol and treated with 20 µM 6-gingerol directly for 2 days, using an Annexin V-FITC & propidium iodide (PI) apoptosis kit. Results are from three independent experiments (n = 3). (f) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel **P < 0.01, ***P < 0.001 (e) . Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.001.
Human Ovarian Carcinoma Cell Line Skov3, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human ovarian carcinoma cell line skov3/product/Procell Inc
Average 90 stars, based on 1 article reviews
human ovarian carcinoma cell line skov3 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

99
ATCC cell line species type purpose skov3 human ovarian carcinoma candidate skbr3 human breast carcinoma candidate c6 rat glioma control
6-gingerol induces apoptosis in <t>SKOV3</t> cells. (a) Clonogenic survival assay showing the survival rates of SKOV3 cells treated with 5 µM,10 µM,15 µM and 20 µM 6-gingerol for different durations (1 st , 2 nd , 4 th , and 6 th days). Results are based on independent experiments (n = 3). (b) Western blot analysis of cleaved caspase-3 or and cleaved PARP levels in SKOV3 cells treated with 6-gingerol. Tubulin was used as the loading control. (c) Flow cytometry analysis of apoptosis in SKOV3 cells treated with different 6-gingerol concentrations, using an Annexin V-FITC & propidium iodide (PI) apoptosis kit. Results are from three independent experiments (n = 3). (d) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel (c) . Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.001. (e) Flow cytometry analysis of apoptosis in SKOV3 cells treated with 20 µM Z-VAD-FMK (Selleck Chemicals) for 2 hours, followed by treatment with 20 µM 6-gingerol and treated with 20 µM 6-gingerol directly for 2 days, using an Annexin V-FITC & propidium iodide (PI) apoptosis kit. Results are from three independent experiments (n = 3). (f) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel **P < 0.01, ***P < 0.001 (e) . Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.001.
Cell Line Species Type Purpose Skov3 Human Ovarian Carcinoma Candidate Skbr3 Human Breast Carcinoma Candidate C6 Rat Glioma Control, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cell line species type purpose skov3 human ovarian carcinoma candidate skbr3 human breast carcinoma candidate c6 rat glioma control/product/ATCC
Average 99 stars, based on 1 article reviews
cell line species type purpose skov3 human ovarian carcinoma candidate skbr3 human breast carcinoma candidate c6 rat glioma control - by Bioz Stars, 2026-03
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90
Procell Inc human ovarian carcinoma cell line skov3 procell cl-0215
6-gingerol induces apoptosis in <t>SKOV3</t> cells. (a) Clonogenic survival assay showing the survival rates of SKOV3 cells treated with 5 µM,10 µM,15 µM and 20 µM 6-gingerol for different durations (1 st , 2 nd , 4 th , and 6 th days). Results are based on independent experiments (n = 3). (b) Western blot analysis of cleaved caspase-3 or and cleaved PARP levels in SKOV3 cells treated with 6-gingerol. Tubulin was used as the loading control. (c) Flow cytometry analysis of apoptosis in SKOV3 cells treated with different 6-gingerol concentrations, using an Annexin V-FITC & propidium iodide (PI) apoptosis kit. Results are from three independent experiments (n = 3). (d) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel (c) . Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.001. (e) Flow cytometry analysis of apoptosis in SKOV3 cells treated with 20 µM Z-VAD-FMK (Selleck Chemicals) for 2 hours, followed by treatment with 20 µM 6-gingerol and treated with 20 µM 6-gingerol directly for 2 days, using an Annexin V-FITC & propidium iodide (PI) apoptosis kit. Results are from three independent experiments (n = 3). (f) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel **P < 0.01, ***P < 0.001 (e) . Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.001.
Human Ovarian Carcinoma Cell Line Skov3 Procell Cl 0215, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human ovarian carcinoma cell line skov3 procell cl-0215/product/Procell Inc
Average 90 stars, based on 1 article reviews
human ovarian carcinoma cell line skov3 procell cl-0215 - by Bioz Stars, 2026-03
90/100 stars
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92
OriGene ovca cell line skov3
6-gingerol induces apoptosis in <t>SKOV3</t> cells. (a) Clonogenic survival assay showing the survival rates of SKOV3 cells treated with 5 µM,10 µM,15 µM and 20 µM 6-gingerol for different durations (1 st , 2 nd , 4 th , and 6 th days). Results are based on independent experiments (n = 3). (b) Western blot analysis of cleaved caspase-3 or and cleaved PARP levels in SKOV3 cells treated with 6-gingerol. Tubulin was used as the loading control. (c) Flow cytometry analysis of apoptosis in SKOV3 cells treated with different 6-gingerol concentrations, using an Annexin V-FITC & propidium iodide (PI) apoptosis kit. Results are from three independent experiments (n = 3). (d) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel (c) . Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.001. (e) Flow cytometry analysis of apoptosis in SKOV3 cells treated with 20 µM Z-VAD-FMK (Selleck Chemicals) for 2 hours, followed by treatment with 20 µM 6-gingerol and treated with 20 µM 6-gingerol directly for 2 days, using an Annexin V-FITC & propidium iodide (PI) apoptosis kit. Results are from three independent experiments (n = 3). (f) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel **P < 0.01, ***P < 0.001 (e) . Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.001.
Ovca Cell Line Skov3, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ovca cell line skov3/product/OriGene
Average 92 stars, based on 1 article reviews
ovca cell line skov3 - by Bioz Stars, 2026-03
92/100 stars
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90
Pasteur Institute human ovarian carcinoma cell lines skov3, 2008c13, ovcar3, and a2780s
6-gingerol induces apoptosis in <t>SKOV3</t> cells. (a) Clonogenic survival assay showing the survival rates of SKOV3 cells treated with 5 µM,10 µM,15 µM and 20 µM 6-gingerol for different durations (1 st , 2 nd , 4 th , and 6 th days). Results are based on independent experiments (n = 3). (b) Western blot analysis of cleaved caspase-3 or and cleaved PARP levels in SKOV3 cells treated with 6-gingerol. Tubulin was used as the loading control. (c) Flow cytometry analysis of apoptosis in SKOV3 cells treated with different 6-gingerol concentrations, using an Annexin V-FITC & propidium iodide (PI) apoptosis kit. Results are from three independent experiments (n = 3). (d) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel (c) . Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.001. (e) Flow cytometry analysis of apoptosis in SKOV3 cells treated with 20 µM Z-VAD-FMK (Selleck Chemicals) for 2 hours, followed by treatment with 20 µM 6-gingerol and treated with 20 µM 6-gingerol directly for 2 days, using an Annexin V-FITC & propidium iodide (PI) apoptosis kit. Results are from three independent experiments (n = 3). (f) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel **P < 0.01, ***P < 0.001 (e) . Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.001.
Human Ovarian Carcinoma Cell Lines Skov3, 2008c13, Ovcar3, And A2780s, supplied by Pasteur Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human ovarian carcinoma cell lines skov3, 2008c13, ovcar3, and a2780s/product/Pasteur Institute
Average 90 stars, based on 1 article reviews
human ovarian carcinoma cell lines skov3, 2008c13, ovcar3, and a2780s - by Bioz Stars, 2026-03
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6-gingerol induces apoptosis in SKOV3 cells. (a) Clonogenic survival assay showing the survival rates of SKOV3 cells treated with 5 µM,10 µM,15 µM and 20 µM 6-gingerol for different durations (1 st , 2 nd , 4 th , and 6 th days). Results are based on independent experiments (n = 3). (b) Western blot analysis of cleaved caspase-3 or and cleaved PARP levels in SKOV3 cells treated with 6-gingerol. Tubulin was used as the loading control. (c) Flow cytometry analysis of apoptosis in SKOV3 cells treated with different 6-gingerol concentrations, using an Annexin V-FITC & propidium iodide (PI) apoptosis kit. Results are from three independent experiments (n = 3). (d) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel (c) . Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.001. (e) Flow cytometry analysis of apoptosis in SKOV3 cells treated with 20 µM Z-VAD-FMK (Selleck Chemicals) for 2 hours, followed by treatment with 20 µM 6-gingerol and treated with 20 µM 6-gingerol directly for 2 days, using an Annexin V-FITC & propidium iodide (PI) apoptosis kit. Results are from three independent experiments (n = 3). (f) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel **P < 0.01, ***P < 0.001 (e) . Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.001.

Journal: Frontiers in Oncology

Article Title: 6-gingerol promotes apoptosis of ovarian cancer cells through miR-506/Gli3 signaling pathway activation

doi: 10.3389/fonc.2025.1547771

Figure Lengend Snippet: 6-gingerol induces apoptosis in SKOV3 cells. (a) Clonogenic survival assay showing the survival rates of SKOV3 cells treated with 5 µM,10 µM,15 µM and 20 µM 6-gingerol for different durations (1 st , 2 nd , 4 th , and 6 th days). Results are based on independent experiments (n = 3). (b) Western blot analysis of cleaved caspase-3 or and cleaved PARP levels in SKOV3 cells treated with 6-gingerol. Tubulin was used as the loading control. (c) Flow cytometry analysis of apoptosis in SKOV3 cells treated with different 6-gingerol concentrations, using an Annexin V-FITC & propidium iodide (PI) apoptosis kit. Results are from three independent experiments (n = 3). (d) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel (c) . Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.001. (e) Flow cytometry analysis of apoptosis in SKOV3 cells treated with 20 µM Z-VAD-FMK (Selleck Chemicals) for 2 hours, followed by treatment with 20 µM 6-gingerol and treated with 20 µM 6-gingerol directly for 2 days, using an Annexin V-FITC & propidium iodide (PI) apoptosis kit. Results are from three independent experiments (n = 3). (f) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel **P < 0.01, ***P < 0.001 (e) . Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.001.

Article Snippet: The SKOV3 human ovarian carcinoma cell line was obtained and authenticated by the American Type Culture Collection (Manassas, VA, USA).

Techniques: Clonogenic Cell Survival Assay, Western Blot, Control, Flow Cytometry

6-gingerol inhibits SKOV3 cells by reducing Gli3 expression. (a) Western blot analysis showing Gli3 protein levels in SKOV3 cells treated with 6-gingerol. Tubulin was used as a loading control. (b) Western blot analysis of apoptosis-related proteins (Bcl-xL, anti-Bcl-2, and Bax) in SKOV3 cells treated with 6-gingerol. Tubulin was used as a loading control.

Journal: Frontiers in Oncology

Article Title: 6-gingerol promotes apoptosis of ovarian cancer cells through miR-506/Gli3 signaling pathway activation

doi: 10.3389/fonc.2025.1547771

Figure Lengend Snippet: 6-gingerol inhibits SKOV3 cells by reducing Gli3 expression. (a) Western blot analysis showing Gli3 protein levels in SKOV3 cells treated with 6-gingerol. Tubulin was used as a loading control. (b) Western blot analysis of apoptosis-related proteins (Bcl-xL, anti-Bcl-2, and Bax) in SKOV3 cells treated with 6-gingerol. Tubulin was used as a loading control.

Article Snippet: The SKOV3 human ovarian carcinoma cell line was obtained and authenticated by the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Western Blot, Control

6-gingerol increases microRNA (miR)-506 expression in SKOV3 cells. RT-PCR analysis showing the expression levels of candidate microRNAs predicted to target Gli3 in SKOV3 cells treated with 6-gingerol. Data are normalized to the levels of 5s rRNA.

Journal: Frontiers in Oncology

Article Title: 6-gingerol promotes apoptosis of ovarian cancer cells through miR-506/Gli3 signaling pathway activation

doi: 10.3389/fonc.2025.1547771

Figure Lengend Snippet: 6-gingerol increases microRNA (miR)-506 expression in SKOV3 cells. RT-PCR analysis showing the expression levels of candidate microRNAs predicted to target Gli3 in SKOV3 cells treated with 6-gingerol. Data are normalized to the levels of 5s rRNA.

Article Snippet: The SKOV3 human ovarian carcinoma cell line was obtained and authenticated by the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

miR-506 suppresses Gli3 and induces apoptosis in SKOV3 cells. (a) Flow cytometry analysis of apoptosis in SKOV3 cells after transfection with miR-506, using Annexin V-FITC and propidium iodide (PI) staining. Results are based on three independent experiments (n = 3). (b) Quantification of apoptotic cells from panel (a) . The data show the percentage of double-positive Annexin V and PI cells. Results are presented as mean ± SD (n = 3). ** P < 0.01. (c) Western blot analysis showing Gli3 protein levels. Tubulin was used as a loading control.

Journal: Frontiers in Oncology

Article Title: 6-gingerol promotes apoptosis of ovarian cancer cells through miR-506/Gli3 signaling pathway activation

doi: 10.3389/fonc.2025.1547771

Figure Lengend Snippet: miR-506 suppresses Gli3 and induces apoptosis in SKOV3 cells. (a) Flow cytometry analysis of apoptosis in SKOV3 cells after transfection with miR-506, using Annexin V-FITC and propidium iodide (PI) staining. Results are based on three independent experiments (n = 3). (b) Quantification of apoptotic cells from panel (a) . The data show the percentage of double-positive Annexin V and PI cells. Results are presented as mean ± SD (n = 3). ** P < 0.01. (c) Western blot analysis showing Gli3 protein levels. Tubulin was used as a loading control.

Article Snippet: The SKOV3 human ovarian carcinoma cell line was obtained and authenticated by the American Type Culture Collection (Manassas, VA, USA).

Techniques: Flow Cytometry, Transfection, Staining, Western Blot, Control

6-gingerol induces apoptosis in SKOV3 cells via miR-506. (a) Clonogenic survival assay showing the percentage of SKOV3 cells surviving after treatment with 20 μM 6-gingerol or 20 μM 6-gingerol + antago-miR-506 over different time points (days 1, 2, 4, 6, and 8). Results are based on three independent experiments (n = 3). (b) Flow cytometry analysis of apoptosis in SKOV3 cells treated with 6-gingerol or 6-gingerol + antago-miR-506 using Annexin V-FITC and propidium iodide (PI) staining (n = 3) # P > 0.05, *** P < 0.001. (c) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel (b) . Results are presented as mean ± SD (n = 3). ***P < 0.001, #P > 0.05. (d) Western blot was performed with anti-Gli3 antibody. Tubulin was used as a loading control.

Journal: Frontiers in Oncology

Article Title: 6-gingerol promotes apoptosis of ovarian cancer cells through miR-506/Gli3 signaling pathway activation

doi: 10.3389/fonc.2025.1547771

Figure Lengend Snippet: 6-gingerol induces apoptosis in SKOV3 cells via miR-506. (a) Clonogenic survival assay showing the percentage of SKOV3 cells surviving after treatment with 20 μM 6-gingerol or 20 μM 6-gingerol + antago-miR-506 over different time points (days 1, 2, 4, 6, and 8). Results are based on three independent experiments (n = 3). (b) Flow cytometry analysis of apoptosis in SKOV3 cells treated with 6-gingerol or 6-gingerol + antago-miR-506 using Annexin V-FITC and propidium iodide (PI) staining (n = 3) # P > 0.05, *** P < 0.001. (c) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel (b) . Results are presented as mean ± SD (n = 3). ***P < 0.001, #P > 0.05. (d) Western blot was performed with anti-Gli3 antibody. Tubulin was used as a loading control.

Article Snippet: The SKOV3 human ovarian carcinoma cell line was obtained and authenticated by the American Type Culture Collection (Manassas, VA, USA).

Techniques: Clonogenic Cell Survival Assay, Flow Cytometry, Staining, Western Blot, Control