Journal: Frontiers in Oncology
Article Title: 6-gingerol promotes apoptosis of ovarian cancer cells through miR-506/Gli3 signaling pathway activation
doi: 10.3389/fonc.2025.1547771
Figure Lengend Snippet: 6-gingerol induces apoptosis in SKOV3 cells. (a) Clonogenic survival assay showing the survival rates of SKOV3 cells treated with 5 µM,10 µM,15 µM and 20 µM 6-gingerol for different durations (1 st , 2 nd , 4 th , and 6 th days). Results are based on independent experiments (n = 3). (b) Western blot analysis of cleaved caspase-3 or and cleaved PARP levels in SKOV3 cells treated with 6-gingerol. Tubulin was used as the loading control. (c) Flow cytometry analysis of apoptosis in SKOV3 cells treated with different 6-gingerol concentrations, using an Annexin V-FITC & propidium iodide (PI) apoptosis kit. Results are from three independent experiments (n = 3). (d) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel (c) . Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.001. (e) Flow cytometry analysis of apoptosis in SKOV3 cells treated with 20 µM Z-VAD-FMK (Selleck Chemicals) for 2 hours, followed by treatment with 20 µM 6-gingerol and treated with 20 µM 6-gingerol directly for 2 days, using an Annexin V-FITC & propidium iodide (PI) apoptosis kit. Results are from three independent experiments (n = 3). (f) Quantification of apoptotic cells (double-positive for PI and Annexin V) from panel **P < 0.01, ***P < 0.001 (e) . Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.001.
Article Snippet: The SKOV3 human ovarian carcinoma cell line was obtained and authenticated by the American Type Culture Collection (Manassas, VA, USA).
Techniques: Clonogenic Cell Survival Assay, Western Blot, Control, Flow Cytometry